Maikulosikopu ya fluorescence ndi mtundu wa microscope ya kuwala yomwe imagwiritsa ntchito gwero lowala kwambiri kuti liwunikire chithunzicho ndi kusangalatsa ma fluorochromes pachitsanzocho. Kuwala kwa chitsanzocho nthawi zambiri kumachitika ndi kuwala komwe kumatulutsa kuwala kwa ultraviolet. Amagwiritsidwa ntchito kwambiri pazachilengedwe, zamankhwala ndi mafakitale.
Kodi microscope ya fluorescence imagwira ntchito bwanji?
Ma microscope a fluorescence amagwiritsa ntchito nyali ya mercury kapena xenon kuti apange kuwala kwa ultraviolet. Kuwala kumabwera mu microscope ndikugunda pagalasi la dichroic - galasi lomwe limawonetsa mtundu umodzi wa kutalika kwa mafunde ndi kulola mtundu wina kudutsa. Magalasi a dichroic amawonetsa kuwala kwa ultraviolet kupita ku chitsanzo. Zitsanzo zina zimakhala ndi fluorescent mwachibadwa pansi pa kuwala kwa ultraviolet chifukwa zimakhala ndi zinthu za fulorosenti monga chlorophyll. Ngati chitsanzocho sichinawonekere mwachibadwa, chikhoza kuipitsidwa ndi utoto wa fulorosenti wotchedwa fluorochromes.
Kodi microscope ya fluorescence imakhala ndi chiyani?
1. Gwero la Kuwala
Magwero owunikira kwambiri ndi mercury, xenon, ndi ma LED. Mercury imapereka kuwala kwabwino kwambiri kwa microscope ya fluorescence. Ma LED akukhala otchuka kwambiri chifukwa ndi otsika mtengo kuposa magwero ena ndipo amagwiritsa ntchito mphamvu zochepa.
| Mwala wa Mercury | Xenon Lamp | LED | |
| Wavelength wa Kuwala Kotulutsidwa | 350-370 nm | 400nm ~ 450nm | 400-700 nm |
| Moyo wonse (ola) | 200-300 | 400-600 | 10000 |
| Ubwino | 1. Kuwala Kwakukulu Kwambiri, Magwero Ambiri Owala Kwambiri. 2. Imatulutsa ultraviolet yamphamvu ndi blue-violet kuti isangalatse mitundu yonse ya fluorophores. 3. Zithunzi zowala komanso zokongola. | 1. The spectral intensity ndi yokhazikika. 2. Kuwoneka kwamphamvu kwamphamvu mu infuraredi ndi pakati pa infrared. | 1. Zosavuta kusintha. 2. Palibe chifukwa chotenthetsa. 3. Ikhoza kuyatsidwa ndi kuzimitsa nthawi yomweyo. 4. Control kuwala gwero mphamvu. |
| Kuipa | 1. Moyo Waufupi. 2. Kutentha nthawi yayitali. | 1. Bokosi lamagetsi lapadera la DC limafunikira pakuwotcha kwambiri. 2. Zokwera mtengo kuposa Nyali ya Mercury | 1. Kuwala kwa UV ndi kofooka kuposa nyali ya mercury. 2.Utali uliwonse wa kuwala umafuna LED yosiyana chifukwa cha mayendedwe opapatiza omwe amatulutsa. |
Kuyika kwa Mercury Fluorescent
Kuyika kwa Fluorescent ya LED
2. Zosefera Zosangalatsa
Fyuluta yosangalatsa ndiyofunikira pakugwira ntchito kwa microscope ya fluorescence. Imadutsa kuwala kwa utali waufupi, womwe utoto wa fulorosenti ukhoza kuyamwa. Komanso, imatchinga magwero ena a kuwala kosangalatsa.
Zosefera Zosangalatsa(B,G,U,V)
3. Dichroic Mirror
Dichroic galasi ndi mtundu wa fyuluta ya kuwala yomwe imawonetsa kuwala pamafunde ena pomwe imatumiza ena. Amagwiritsidwa ntchito mu microscopes ya fluorescence kuti alekanitse chisangalalo ndi mafunde otulutsa.
4. Sefa yotulutsa
Fyuluta yotulutsa mpweya imadutsa mafunde okhawo opangidwa ndi fluorophore ndikutchinga kuwala konse kosafunika kunja kwa gululi - makamaka mafunde osangalatsa.
5. Mitundu ya fluorescent
Utoto wa fulorosenti ndi zinthu zakuthupi zomwe zimakhala ndi fluorescence, zomwe zimatha kupanga chithunzi cha fulorosenti potulutsa kuwala kobiriwira kowoneka bwino kowoneka bwino pambuyo posangalala ndi kuwala kowala kwambiri kwa ultraviolet. Mitundu yomwe imagwiritsidwa ntchito kwambiri ndi fulorosenti ndi; DAPI (49,6-diamidino-2-phenylindole), acridine orange, auramine-rhodamine, Alexa Fluors, kapena DyLight 488.
Ndi mitundu ingati ya ma microscopes a fluorescence?
1. Maikulosikopu Yolunjika Epifluorescence:
Ndiwo mtundu wodziwika bwino wa microscope wa fluorescence. Kusangalatsa kwa fluorophore ndi kuzindikira kwa fluorescence kumachitika kudzera munjira yowala yofanana (ie kudzera mu cholinga). Zambiri mwa ma microscopes a fluorescence, makamaka omwe amagwiritsidwa ntchito mu sayansi ya moyo, ndi mapangidwe a epifluorescence.
BS-2081F Research Fluorescent Biological microscope
2. Maikulosikopu ya Confocal Fluorescence:
Confocal Fluorescence Maikulosikopu: Mtundu uwu wa maikulosikopu wa fluorescence umaphatikiza kusanthula kwa laser ndi kuwala kwa fulorosenti kuti apange chithunzi. Itha kugwiritsidwa ntchito pazinthu zosiyanasiyana, monga kuphunzira ma cell ndi minofu, kuzindikira mapuloteni ndi zinthu zina m'maselo, komanso kuyeza makulidwe azinthu.
BS-2081F Research Fluorescent Biological microscope
3. Maikulosikopu ya Fluorescence Inverted
Gwero la kuwala kwa maikulosikopu ndi condenser zamtunduwu zili pamwamba, moyang'ana pansi. Kuwala kounikira kuyenera kukhala madigiri 90 molingana ndi pamwamba pa chithunzi chomwe chikuwunikiridwa.
BS-2095F Maikulosikopu ya Fluorescent Inverted
Kodi ubwino ndi kuipa kwa maikulosikopu a fulorosenti ndi chiyani?
Ubwino:
- Imathandiza kuwona ma cell a zamoyo popanda kuwawononga
- Amapereka zithunzi zowoneka bwino zokhala ndi mitundu yolondola
- Imalola kuphunzira njira zamoyo m'maselo
- Gwiritsani ntchito kuzindikira mitundu yosiyanasiyana ya mamolekyu mkati mwa maselo, monga mapuloteni kapena ma nucleic acid.
Zoyipa:
-Imangolola kuwona zinthu zina mkati mwa cell yomwe ili ndi utoto wa fulorosenti.
-The photobleaching chifukwa cha chisangalalo cha elekitironi panthawi ya fulorosenti zingakhudze mamolekyu ogwira ntchito a utoto wa fulorosenti. Zotsatira zake, utoto wokhazikikawo ukhoza kutaya mphamvu yake ya mankhwala a fulorosenti.
-Maselo amatha kutengeka ndi phototoxic zotsatira pambuyo podetsedwa ndi utoto wa fulorosenti, monga momwe mamolekyu a fluorophore amatenga ma photon amphamvu kwambiri kuchokera ku kuwala kwaufupi-wavelength.
Kodi ma microscope a fluorescence amagwiritsa ntchito chiyani?
Ma microscopes a fluorescence amagwiritsidwa ntchito kwambiri m'magawo osiyanasiyana ofufuza ndi kugwiritsa ntchito kuphatikiza biochemistry, cell biology, microbiology, immunology, ndi zamankhwala.
1. Pankhani ya biology, microscope ya fluorescence imathandizira kuzindikira molondola komanso mwatsatanetsatane zigawo za ma cell ndi submicroscopic ma cell ndi ntchito mothandizidwa ndi kulemba utoto wa fulorosenti.
2. Zachipatala, maikulosikopu a fluorescence amatha kugwiritsa ntchito mankhwala opangira fulorosenti kuti azindikire kupezeka ndi kufalikira kwa mabakiteriya ndi mavairasi, kapena kuthandizira kulemba zolinga za opaleshoni kuti athandize opaleshoni.
3. Pankhani ya mineralogy, microscope ya fluorescence nthawi zambiri imagwiritsidwa ntchito pophunzira zinthu zomwe zimakhala ndi fluorescence zokha, monga asphalt, petroleum, malasha, graphene oxide ndi mchere wina.
4. Mu sayansi yazinthu, ma microscope a fluorescence angagwiritsidwe ntchito m'makampani opanga nsalu kapena makampani opanga mapepala kuti asanthule zinthu zopangidwa ndi ulusi.
Nthawi yotumiza: Mar-14-2023